PERTUMBUHAN BAWANG MERAH (Allium ascalonicum L.) DARI BENIH PADA BERBAGAI KONSENTRASI SUKROSA DAN KEPEKATAN MEDIA MURASHIGE DAN SKOOG
Article History
Submited : December 27, 2022
Published : December 1, 2022
The growth of explants in tissue culture media is determined by a number of factors, including the concentration of sucrose and the concentration of nutrients in the media used. The experiment was designed to determine the suitable concentration of sucrose and nutrient concentration in MS media for the growth of shallot from seeds. The experiment used a Two-factorial Completely Randomized design (CRD), namely the concentration of sucrose (30 g sucrose/l MS media, 45 g sucrose/l MS media, and 60 g sucrose/l MS media) and the concentration of MS media nutrients (0.5 times, 1 times, and 1.5 times the concentration). There were nine combinations of treatments, with each treatment repeated four times. A total of 108 shallot seeds were used in the experiment, with each experimental unit consisting of three shallot seed explants. The observed variables were the time when buds appeared, the number of leaves, the length of the leaves, and the weight of biomass. The results of the experiment showed that the concentration of 30 g sucrose/l media and 0.5 times concentration of the basic media MS is the most suitable composition for the initial growth of shallots from seeds. This was indicated by the fastest buds and the formation of the most leaves at 2 weeks after planting. The use of sucrose or MS base media, each at a concentration of 30 g sucrose/l Media or 0.5 times of concentration, was found to be suitable for the growth of shallots indicated by the longest leaf growth and the heaviest biomass.
Bioteknologi Pertanian - Google Books. (n.d.). Retrieved August 6, 2021.
Bollivar, D.W., 2006. Recent Advance in Chlorophyll Biosynthesis. Photosynthesis Research. vol 89(3): 1-22.
Dasar Teknik Kultur Jaringan Tanaman - Google Books. (n.d.). Retrieved August 7, 2021.
Fosket, D.E., 1994. Plant Growth and Development: a Molecular Approach. Academic Press, San Diego.
Hopkins, W.G, 1999. Introduction to Plant Physiologi. John Willey and Sons Inc., New York. Pg 98-180.
Hepler, P.K. and Winship, L.J., 2010. Calcium at the Cell Wall-Cytoplast Interface. Journal of Integrative Plant Biology. vol 52(2): 147-160.
Hasan, A. H., Sekolah, B., Penyuluhan, T., & Medan, P. 2014. Kajian Pemanfaatan Kultur Jaringan Dalam Perbanyakan Tanaman Bebas Virus.
Handayani, E., Basri, Z. dan Maemunah, 2020. Pertumbuhan Bawang Merah dari Biji pada Berbagai Kepekatan Garam Mineral Media MS dan Konsentrasi BAP. Mitra Sains. vol 8(2): 183-198.
Irfan Kepala Lab Patologi, M., & dan Mikrobiologi Fak Pertanian dan Peternakan UIN Suska Riau, E. (2013). Respon Bawang Merah (Allium ascalonicum L) Terhadap Zat Pengatur Tumbuh dan Unsur Hara. Jurnal Agroteknologi. vol 3(2): 35-40.
Jo, E.A., Tewari, R.K., Hahn, E.J. and Paek, K.Y., 2009. In Vitro Sucrose Concentration affects growth and Acclimatization of Alocasia Amazonica Plantlets. Plant Cell Tissue and Organ Culture. vol 96(3): 307-315.
Kasim, H., Yusran, and Basri, Z. 2015. The Strength of MS Media and Steriliszation Technique on Red Dragonfruit (Hylocereus polyrhizus) Seed Germination. J. Agroland. vol 2 (1): 33-40.
Kultur Jaringan Anggrek Skala Rumah Tangga - Ir. Edhi Sandra - Google Buku. (n.d.). Retrieved August 6, 2021.
Kultur Jaringan Nanas - Google Books. (n.d.). Retrieved August 7, 2021.
Kultur Jaringan Skala Rumah Tangga - Eka Nurwulan Asriani - Google Buku. (n.d.). Retrieved August 7, 2021.
Kultur Jaringan Tanaman Skala Rumah Tangga - Nurheti Yuliarti - Google Buku. (n.d.). Retrieved August 6, 2021.
Kurnianingsih, R., Ghazali, M., Rosidah, S., Muspiah, A., Astuti, S. P., & Nikmatullah,A. (2020). Pelatihan Teknik Dasar Kultur Jaringan Tumbuhan. JMM (Jurnal Mayarakat Mandiri). vol 4(5): 888-896.
Leghari, S.J., Wahocho, N.A. and Abdul, G.M., 2016. Role of Nitrogen for Plant Growth and Development : a Review, Advance in Environmental Biology. vol 10(9): 209-219.
Lemoine, R. 2000. Sucrose Transporters in Plants: Function and Structure. Biochimica et Biophysica Acta. vol 1465(2): 246-262.
Murashige., T and F. Skoog. 1962. A Revised Medium for Rapid Growth and Biosasays with Tobacco Tissue Culture. Physiol Plant. 15:473-497. (Leucaena leucocephala cv. Tarramba) terhadap cekaman kemasaman media dengan level pemberian aluminium melalui kultur jaringan. Jurnal Sain Peternakan Indonesia. vol 12(1): 71-82.
Novianta, K. I., Sritamin, M., & Adiartayasa, I. W. (2019). Organogenesis Tanaman Bawang Merah (Allium ascalonicum L .) Menggunakan Umbi Secara In Vitro pada Media Dasar Murashige and Skoog yang Diperkaya Vitamin B5 dengan Naftalene Acetic Acid dan 6 -Benzyl Amino Purine. Jurnal Agroekoteknologi Tropika. vol 8(3): 302-310.
Ni’mah, F., Ratnasari, E. dan Budipratama, L.S., 2012. Pengaruh Pemberian Berbagai Kombinasi Konsentrasi Sukrosa dan Kinetin terhadap Induksi Umbi Mikro Kentang (Solanum Tuberosum L.) Kultivar Granola Kembang secara in Vitro. Lentera Bio. vol 1(1): 41-48.
Nurjanani, Lamba, S.E., Ramlan, Ruchjaniningsih, Taufik, M., Maintang dan F. Djufry, 2015. Pengembangan Benih Sumber True Shallot Seed (TSS) dan Umbi Mini Bawang Merah serta Pembinaan Petani Penangkar Benih Bawang Merah di Kabupaten Jenepontoh. Makassar. Balitbangda Provinsi Sulawesi Selatan.
Novianti, A. K. P., Bustami, M. U., & Basri, Z. (2014). Sterilisasi dan Induksi Kalus Bawang Merah (Allium Ascalonicum L.) Lokal Palu secara In Vitro. E-Journal Agrotekbis. vol 2 (2) : 129-137.
Ornay, A.T., 2019. Budidaya Bawang Merah asal Biji. Kementerian Pertanian RI, Jakarta.
Pengaruh konsentrasi sukrosa dan zat pengatur tumbuh sitokinin pada perbanyakan Tanaman Bawang Merah (Allium cepa L.). - Google Books. (n.d.). Retrieved August 7, 2021.
Sunaryo, 2019. Cara Tanam Bawang Merah dari Biji. Kementerian Pertanian RI, Jakarta.
Serfina, R., Mukarlina dan Riza L. 2017. Respon Pertumbuhan Tunas Lidah Buaya (Alloe barbadensi Mill.) dengan Penambahan Ekstrak Touge dan BAP (Benzylamino Purine). Protobiont vol 6 (3):142-146.
Setiti, Sripuji dan Soedarto, 1997. Peranan Media dan Zat Pengatur Tumbuh Untuk Diferensiasi dan Induksi Kalus Pada Budidaya Jaringan Melon. Jurnal Hortikultura. vol 7 (4): 153-157.
Taiz, L. and Zeiger, E., 2010. Plant Physiology. 5th Edition. Sinauer Associates Inc. Publishers, Massachusetts, USA.
Zulkarnain. (2011). Kultur Jaringan Tanaman.pdf (p. 250).